|
KMID : 0368420000430030179
|
|
Journal of Plant Biology
2000 Volume.43 No. 3 p.179 ~ p.182
|
|
|
Antibiotics Resistance of a Red Alga, Griffithsia japonica
|
|
Lee, Yoo Kyung
An, Gynheung/Lee, In Kyu
|
|
|
Abstract
|
|
|
|
To identify antibiotics suitable for stable transformation, we tested the resistance of a red alga, Griffithsia japonica Okamura, to four commonly used antibiotics. Very young germlings, with 1∼3 cells, that germinated from the tetraspores were cultured for 40 d in a half PES medium containing kanamycin, streptomycin, hygromycin B, or phleomycin. G. japonica was highly sensitive to 1㎍ mL^-1 of phleomycin and 50㎍ mL^-1 of hygromycin B. However, it was resistant to kanamycin and low levels of streptomycin and hygromycin B. These results suggest that resistance genes for phleomycin or hygromycin B can be used as selectable markers for transformation of C. japonica.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|